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. 2017 Mar 24;7:45300. doi: 10.1038/srep45300

Figure 5. Androgen-induced CREB3L4 expression is regulated by IRE1α pathway.

Figure 5

(a) LNCaP cells were transfected with scrambled or siIRE1α. After 24 hr, the cells were treated with 10-nM R1881 or DMSO/EtOH control in 5% CT-FBS-containing medium, for 24 hr. Whole cell lysates (40 μg) was resolved by SDS/PAGE and immunoblotted for the respective proteins indicated, with GAPDH used as a loading control. (b) LNCaP cells were transfected with scrambled or siIRE1α with hCREBL4N overexpression vector. 48 hr after transfection, microscopic analysis of the cells was performed (x10, upper panel). Western blot was performed to confirm the expression of IRE1α and CREB3L4 expression (lower panel). (c) LNCaP cells were transfected with siIRE1α and/or siCREB3L4 in the presence or absence of R1881 (10-nM). At 48 hr after transfection, microscopic analysis of the cells was performed (x20) (d), and analyzed by western blot, to confirm the expression of PSA, IRE1α, and CREB3L4, with GAPDH as a loading control. (e) Proposed mechanism of action of CREB3L4 in prostate cancer progression.