Figure 2. 3D visualization of representative pluripotent stem cells and CMs.

(a) 3D volumes of intensity distributions of hiPSCs (n=4 colonies), hiPSC-derived CMs (n=4 monolayers) and adult rat ventricular CMs (n=2 cells) for selected bands: 1,008 cm⁻¹ for cell cytoplasm (blue), 789 cm⁻¹ for cell nucleus (red), 2,857 cm⁻¹ for lipids (green), 485 cm⁻¹ for glycogen (white) and a merge of all components. (b) Representative Raman spectra of each subcellular component (cytoplasm (blue), nucleus (red), lipids (green), glycogen (black)), indicating the band used to reconstruct the 3D intensity volumes. Scale bar, 10 μm.