Figure 3. Decreased expression of the glycolytic and lipogenic genes in Zbtb20-deficient liver.

Male control or LZB20KO mice at the age of 3–4 months were fed on normal chow. n=5 per group. (a–e) The mRNA expression levels of the genes involved in glycolysis (a), lipogenesis (b), sterol synthesis and cholesterol metabolism (c), TG secretion and fatty acid oxidation (d) and transcriptional regulators of lipid metabolism (e) were determined in the liver by quantitative RT-PCR with 36B4 mRNA as internal control. (f,g) Western blot analysis for protein levels of ChREBP, Mlx and SREBP in the whole lysate (f) and nuclear extract (g) of the liver. pSREBP and nSREBP indicate precursor and nuclear SREBP, respectively. Tubulin and Lamin A/C were used as internal controls for the whole lysates and nuclear extracts, respectively. A representative western blot for three independent experiments was shown with the quantification plot based on scanning densitometry analysis. Lack of cytoplasmic protein contamination was confirmed in the nuclear extracts by probing tubulin (Supplementary Fig. 13). Data are represented as mean±s.e.m. *P<0.05; **P<0.01 versus control (Student's t-test).