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. 2017 Mar 22;8:14824. doi: 10.1038/ncomms14824

Figure 4. Zbtb20 regulates hepatic lipogenesis partially through ChREBP.

Figure 4

(a) Zbtb20 binds to Scd1 and ChREBP-α promoters in the liver. Liver from normal chow-fed wild-type mice was subjected to ChIP analysis in the fed state. Values represent relative increase of real-time PCR signals for ChIP with anti-ZBTB20 antibodies compared to that for ChIP with control IgG. n=4 per group. (b) ZBTB20 overexpression enhanced the transcriptional activity of the ChREBP-α promoter in Zbtb20-deficient hepatocytes. The luciferase activity was detected 48 h after plasmid transfection, and normalized by the internal control RL-TK. The experiment was repeated three times independently. (c,d) ZBTB20 overexpression induced the glycolytic and lipogenic genes partially through ChREBP in LZB20KO liver. 3-4 month-old male LZB20KO mice were i.v. injected with shRNA-expressing adenoviruses together with Ad-ZBTB20 or Ad-GFP. Five days later, the gene expression was detected in the liver by western blot (c) and qRT-PCR (d). n=5 per group. Data are represented as mean±s.e.m. *P<0.05 versus control or indicated groups (Student's t-test).