Figure 5.

Binding of multivalent SAv constructs to densely biotinylated surfaces. Shown are QCM-D responses recorded during the binding of SAv constructs, and subsequently b-ZZ, to b10%-SAM (A) and b5%-SLB (B). Insets in A and B illustrate the surfaces and molecules used, and the schematic representations in C illustrate the binding features—residual valency, reported by b-ZZ, and orientation—of the different SAv constructs. Data are marked with distinct symbols for each SAv construct: rSAv (squares), tSAv (circles), dSAv-cis (upward-pointing triangles) and dSAv-trans (downward-pointing triangles).