Figure 2. Depletion of FMNL2 and -3 modifies efficiency of lamellipodium protrusion.

Average protrusion velocities of B16-F1 cells upon knockdown of FMNL3 alone (a–c) or two alternative approaches of simultaneous knockdown of FMNL2 and FMNL3, either using one plasmid capable of knocking down expression of both proteins (FMNL2/3 RNAi; d–f) or two plasmids targeting both messages specifically (FMNL2+FMNL3 RNAi; g–i). (a,d,g) Box and whiskers plots with median values given in red, boxes including 50% (25–75%) and whiskers 80% (10–90%) of all measurements; outliers are shown as dots. n, number of cells analysed. Measurements were aided by assembling kymographs (b,e,h) of phase contrast movies. Knockdown efficacies were documented for each individual experiment, with representative western blots shown in c,f,i. Tubulin was used as loading control. ***p<0.001 by Mann–Whitney rank sum test.