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. 2017 Mar 22;8:14832. doi: 10.1038/ncomms14832

Figure 4. Knockout of FMNL2/3 recapitulates RNAi phenotypes.

Figure 4

(a) CRISPR/Cas9-mediated, specific loss of protein expression as documented by western blotting, using FMNL2/3-reactive antibody and tubulin as loading control. Asterisk marks non-specific band. (b) Representative cells stained for the actin cytoskeleton with phalloidin harbouring genotypes as indicated. Bar, 10 μm. (c–e) Box and whiskers plots summarizing the results from respective quantifications of lamellipodium width, microspike number per cell and F-actin intensity done in analogy to what is shown in Fig. 3. (f) Immunolabelling of the Arp2/3 complex subunit p16A (ARPC5A) in B16 cells and FMNL2/3 KO cells as indicated. Scale bar, 10 μm. (g) Quantification of Arp2/3 complex intensities in lamellipodia of wild-type and FMNL2/3 KO cells. ***p<0.001 by Mann–Whitney rank sum test.