Figure 3.

STY-BODIPY serves as the signal carrier in liposome oxidations (A), enabling determination of rate constants (k_inh) and stoichiometries (n) for reactions of inhibitors with chain-carrying peroxyl radicals (B). Average inhibition rate constants and stoichiometry summarized in panel C. Coautoxidations of egg phosphatidylcholine lipids (1 mM) and STY-BODIPY (8 μM) suspended in phosphate-buffered saline (10 mM) at pH 7.4 initiated by MeOAMVN (0.2 mM) at 37 °C (black trace) and inhibited by 2 μM (red trace), 3 μM (green trace), and 4 μM (blue trace) of Lip-1 (D), Fer-1 (E), α-TOH (F), C₁₅-THN (G), and PMHC (H). Reaction progress was monitored by absorbance at 565 nm (ε = 123,676 M^–1 cm^–1). ^aCould not be determined and is assumed to be 2.0.