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. 2017 Mar 7;3(3):232–243. doi: 10.1021/acscentsci.7b00028

Figure 5.

Figure 5

(A) Antiferroptotic activity of the THNs in mouse embryonic fibroblasts. Ferroptosis was initiated by tamoxifen-induced Cre-mediated deletion of the gpx4 gene, and cell survival was determined 6 h postinduction by AquaBluer assay. Corresponding data obtained for Fer-1, Lip-1, and α-TOH are also shown. (B) The THNs prevent glutamate-induced cell death in mouse HT22 hippocampal cells. Cells were treated with 5 mM glutamate and 100 nM test compound for 10 h, after which cell viability was assessed by AquaBluer assay. Corresponding data obtained for Fer-1, Lip-1, α-TOH, and β-mercaptoethanol (β-Me) are also shown. Cell viability was associated with inhibition of lipid peroxidation, as revealed by suppression of BODIPY-C11581/591 oxidation.