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. 2017 Mar 3;3(3):224–231. doi: 10.1021/acscentsci.7b00019

Scheme 2. Conjugation of B. suis Biovar 2 OPS to Tetanus Toxoid.

Scheme 2

Conditions: (a) 10 mM NaIO4, 50 mM NaOAc, pH 5.5, 4 °C 1 h; (b) 0.5 M NH4Cl, 0.1 M NaCNBH3, 37 °C; (c) aminated OPS 5 mg/mL in PBS containing 10% DMSO and 5 mg/mL disuccinimidal glutarate (dsg) 45 min, rt; (d) activated OPS, tetanus toxoid 2.5 mg/mL PBS.