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. 2017 Mar 24;7:45034. doi: 10.1038/srep45034

Figure 3. Length of semen storage alters SE-associated AChE enzymatic activity.

Figure 3

Acetylcholine-esterase (AChE) specific enzyme activity was measured in SE as described in “Methods” where vehicle is PBS. (a) Baseline acetylcholine-esterase activity taken at time = 0 minutes for individual donor and pooled SE. (b) Average baseline AChE enzymatic activity at time = 0 for prolonged (n = 5) and short-term freezing (n = 13). (c) Average baseline AChE enzymatic activity for illicit drug use (n = 3) and no illicit drug use (n = 15). (d) Time course of AChE activity at time = 0, 15, and 30 minutes. Statistics was based on comparing baseline AChE activity to vehicle, short to prolong, and Drug− to Drug+ respectively for panels a, b, and c. For panel D, statistics time values from point 15 and 30 minutes were compared to the 0 time point. Significance was determined by student’s t test. Differences with p values of 0.05 or less are considered significant *p < 0.05, **p < 0.001. Error bars are SEM. ns = not significant.