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. 2017 Mar 24;7:45036. doi: 10.1038/srep45036

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Copyright © 2017, The Author(s)

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(A) Confocal images of the Endothelial Surface Layer (ESL) stained with Alexa488-conjugated wheat germ agglutinin, with views in a horizontal (XY, upper left), and two vertical planes (XZ, lower left; YZ, middle panel), along with a 3D rendering of the imaged channel (right panel). Scale bar is 10 μm. (B) XY view of the ESL after image deconvolution by the microscope point spread function (upper panel), and intensity profile, measured along the yellow line drawn on the upper panel, from which the ESL thickness is measured as the full width at half maximum. (C) Histogram of the ESL thickness, yielding a thickness of 670 ± 200 nm over 140 measurements. (D) ESL thickness measured at 29 different locations along the 100 μm-long image section shown in inset, illustrating the spatial homogeneity of the ESL.