Table 2. Tests of greater difference between the subspecies in contact versus allopatric samples for the relative intensity of each mass peak.
| Females | Males | Both sexes combined | Candidate (A, R)†† | |
|---|---|---|---|---|
| (a) musculus only expression‡ | ||||
| 18575 Da | z = 2.22, p = 0.013 | z = 2.55, p = 0.005 | z = 3.07, p = 0.001* | A, R |
| 18679 Da | z = −4.75, p > 0.9999 | z = −4.74, p > 0.9999 | z = −6.60, p > 0.9999 | A |
| 18752 Da | z = 1.55, p = 0.06 | z = −0.74, p = 0.62 | z = −0.46, p = 0.67 | A |
| (b) domesticus only expression‡ | ||||
| 18651 Da | X2 = 32.65, p < 0.0001* | X2 = 21.03, p < 0.0001* | X2 = 53.90, p < 0.0001* | R |
| 18682 Da | z = 1.93, p = 0.027 | z = −0.58, p = 0.71 | z = 0.57, p = 0.29 | A |
| 18724 Da | z = −4.05, p > 0.9999 | z = −1.64, p = 0.95 | z = −3.74, p > 0.9999 | A |
| (c) masses expressed by both subspecies‡‡ | ||||
| 18666 Da | z = 6.14, p < 0.0001* | z = 3.31, p = 0.0005* | z = 6.81, p < 0.0001* | A, R |
| 18694 Da | z = −3.96, p > 0.9999 | z = −1.13, p = 0.87 | z = −3.35, p = 0.9996 | — |
| 18708 Da | z = 4.44, p < 0.0001* | z = 2.20, p = 0.014 | z = 4.87, p < 0.0001* | A, R |
| 18711 Da | z = 5.27, p < 0.0001* | z = 3.72, p = 0.0001* | z = 6.41, p < 0.0001* | R |
| 18731 Da | z = 6.64, p < 0.0001* | z = 4.15, p < 0.0001* | z = 7.80, p < 0.0001* | A, R |
Frequencies of expression for each subspecies, sex and origin are given in Supplementary Table S1. Divergence between subspecies is examined in Table 1 for each sex and origin (contact/allopatric).
‡For masses expressed by only one subspecies (a, b), the directional prediction of stronger expression in contact versus allopatric samples is assessed by Mann-Whitney U tests, or by contingency chi-squared (when a mass peak was observed in only contact or allopatric samples).
‡‡For masses expressed by both subspecies (c), the directional prediction of stronger subspecies divergence in contact versus allopatric populations was tested using this specific a priori contrast for the interaction between subspecies and origin in a 2 × 2 factorial non-parametric ANOVA.
††A: assortment: subspecies differences evident both in allopatry and contact; R: reinforcement: subspecies differences more marked in contact than in allopatry.
*Significant after a sequential Bonferroni correction.