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. 2017 Mar 24;7:44976. doi: 10.1038/srep44976

Figure 7. Sept9_i2 overexpression induces a loss of septin associated actin filament that is dependent on its N-terminal sequence.

Figure 7

(a) SKBr3 cells were transfected either with control siRNA or siRNA targeting Sept9#2. After Sept9 knockdown, the number of cells with subnuclear long septin filaments associated with actin fibers decreased significantly as shown in the bar graph. Results are from the observation of the number of cells indicated at the bottom of the graph and collected in a duplicate, mean ± S.D.; t-test, **p value < 0.01. (b) SKBr3 cells were transfected either with GFP-Sept9_δN or GFP-Sept9_i2, and with or without the Sept9#2 siRNA. The number of cells with subnuclear long septin filaments associated with actin fibers decreased significantly when GFP- Sept9_δN was present. Results are from the observation of the number of cells indicated at the bottom of the graph and collected in a duplicate, mean ± S.D.; t-test, *p value < 0.05. Image panel display cells representative of cells expressing either GFP-Sept9_δN or GFP-Sept9_i2. Framed regions are zoomed at the bottom of each image. White bars correspond to 10 μm and 5 μm in image and zoomed regions, respectively.