Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 24;7:44976. doi: 10.1038/srep44976

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) SKBr3 cell lysates were incubated with either paclitaxel or phalloidin to polymerize tubulin or G-actin, respectively. After high-speed centrifugation through a sucrose cushion, supernatant and pellet proteins were separated and equivalent amounts of each fraction were analyzed by Western blotting with the indicated antibodies and distribution of each protein in pellets (P) and supernatants (S) was quantified. The percentage of each analyzed protein in the pellets was compared to (b) the percentage of tubulin present in the paclitaxel-microtubule pellets or to (c) the percentage of actin present in the phalloidin-F-actin pellets. Results are from three independent experiments, mean ± S.D.; t-test, *p value < 0.05, **p value < 0.01, ***p value < 0.001. Blot images in panels a) were cropped, full blot images are presented in Supplementary Fig. S8.