Mass accuracy
Electrospray survey
Attribute	Value
Perform lock spray calibration	Yes
Lock spray lock mass	785.8427 Da/e
Lock spray scan	10
Lock mass tolerance	0.1 Da
MSMS
Attribute	Value
Perform lock spray calibration	Yes
Lock spray lock mass	785.8427 Da/e
Lock spray scan	10
Lock mass tolerance	0.1 Da
Noise reduction
Electrospray survey
Attribute	Value
Background subtraction type	Normal
Background threshold	35%
Background polynomial	5
Perform smoothing	Yes
Smoothing type	Savitzky-Golay
Smoothing iterations	2
Smoothing window	3 channels
MSMS
Attribute	Value
Background subtraction type	Normal
Background threshold	35%
Background polynomial	5
Perform smoothing	Yes
Smoothing type	Savitzky-Golay
Smoothing iterations	2
Smoothing window	3 channels
Peptide filter
De-isotoping and centroiding
Electrospray survey
Attribute	Value
Perform de-isotoping	Yes
De-isotoping type	Fast
Iterations	30
Automatic thresholds	No
Threshold	3%
Minimum peak width	4 channels
Centroid top	80%
TOF resolution	10,000
NP multiplier	0.7
MSMS
Attribute	Value
Perform de-isotoping	Yes
De-isotoping type	Fast
Iterations	30
Automatic thresholds	No
Threshold	3%
Minimum peak width	4 channels
Centroid top	80%
TOF resolution	10,000
NP multiplier	0.7