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. 2017 Mar 24;7:43107. doi: 10.1038/srep43107

Figure 10. Effects of PKCγ siRNA on AT1R, PKCγ, GTP-Rac1 (Pull-down Assay) and Cu/Zn-SOD proteins expression, and effects of LOS on AT1R, PKCγ and Cu/Zn-SOD mRNA level in the PVN of salt-induced hypertensive rats.

Figure 10

High salt (HS) intake rats had higher levels of AT1R, PKCγ and GTP-Rac1, but lower level of Cu/Zn-SOD compare with control rats. Bilateral PVN infusion of PKCγ siRNA for 14 days attenuated expression of AT1R, PKCγ and GTP-Rac1, and augmented expression of Cu/Zn-SOD in hypertensive rats. However, admiration of PKCγ siRNA in PVN had no effect on PVN levels of AT1R in salt-induced hypertension. (A) A representative immunoblot; (B) PVN tissues were analyzed for Rac1 activity (Rac1-GTP) as described in METHODS. As controls, protein levels of Rac1 (total-Rac1) in PVN were measured. (C)densitometric analysis of protein expression of AT1R, PKCγ and Cu/Zn-SOD in the PVN in different groups; (D) Densitometric analysis of mRNA expression of AT1R, PKCγ and Cu/Zn-SOD in the PVN in different groups. Values are expressed as means ± SE. *P < 0.05 vs NS groups (NS + PKCγ siRNA or NS + scrambled siRNA); †P < 0.05 HS + PKCγ siRNA vs HS + scrambled siRNA. #P < 0.05 NS + PKCγ siRNA vs NS + scrambled siRNA.