Skip to main content
. 2017 Mar 24;7:42928. doi: 10.1038/srep42928

Figure 1. TrxR1 overexpression enhances the radioresistance of glioma cells.

Figure 1

(a) Representative images of invaded U-87MG and T98G cells stably expressing pcDNA3.1 or pcDNA3.1-TrxR1. Cells migrated to the bottom chamber were stained with crystal violet and observed under a microscope (scale bar = 200 μm). (b) Invasion was quantified by determining the total number of cells that had migrated through the membrane. Accompanying statistical plots were presented to indicate statistical significance between pcDNA3.1 transfected cells and pcDNA3.1-TrxR1 transfected cells. *p < 0.05, U-87MG cells, #p < 0.05, T98G cells. (c,d) U-87MG and T98G cells were treated with 8 Gy irradiation 2 h before extraction, and the expression levels of TrxR1 and TIGAR was examined by Western blot (*p < 0.05, #p < 0.05). (e,f) Clonogenic capacity of U-87MG and T98G cells with or without TrxR1 overexpression was investigated after a range of radiation doses. *p < 0.05, pcDNA3.1 vs. pcDNA3.1-TrxR1.