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. 2017 Mar 24;7:42928. doi: 10.1038/srep42928

Figure 2. TIGAR interfering results in radiosensitization of glioma cells with TrxR1 overexpression.

Figure 2

(a,b) Representative images of invaded U-87MG and T98G cells (scale bar = 200 μm). Cells overexpressing TrxR1 were treated with TIGAR siRNAs 48 h before inoculating into the matrigel. Accompanying statistical plots were presented to indicate statistical significance. *p < 0.05, pcDNA3.1+ scramble siRNA vs. pcDNA3.1-TrxR1+ scramble siRNA, #p < 0.05, pcDNA3.1+ TIGAR siRNA1 vs. pcDNA3.1-TrxR1+ TIGAR siRNA1, $p < 0.05, pcDNA3.1+ TIGAR siRNA2 vs. pcDNA3.1-TrxR1+ TIGAR siRNA2. (c,d) Clonogenic capacity of TrxR1-overexpressing U-87MG and T98G cells. Scramble (Scr.) or TIGAR siRNA transfection was performed 48 h before irradiation. *p < 0.05, pcDNA3.1-TrxR1+ Scr. siRNA vs. pcDNA3.1-TrxR1+ TIGAR siRNA, #p < 0.05, pcDNA3.1+ Scr. siRNA vs. pcDNA3.1-TrxR1+ TIGAR siRNA. (e,f) Western blot analysis of protein expression levels in U-87MG and T98G cells. Cells were transfected with TIGAR siRNA 48 h before IR and underwent 8-Gy irradiation 2 h before being extracted.