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. 2017 Mar 24;7:42928. doi: 10.1038/srep42928

Figure 3. TIGAR knockdown observably abolishes IR-induced Trx1 nuclear translocation in TrxR1-overexpressing glioma cells.

Figure 3

(a,b) Western blot analysis for nuclear lysates and whole cell lyastes of U-87MG and T98G cells. Cells were extracted 2 h post sham irradiation or 8-Gy IR. (c,d) U-87MG and T98G cells were transfected with TIGAR siRNA1 48 h before IR and underwent 8-Gy irradiation 2 h before being extracted. The expression levels of nuclear Trx1 and total Trx1 were examined by Western blot. (e,f) Western blot analysis for the cell lysates of Trx1-mutant U-87MG and T98G cells. Cells were transfected with pcDNA3.1 or pcDNA3.1-TrxR1 48 h before IR and underwent 8-Gy irradiation 2 h before being extracted. (g,h) Clonogenic capacity of mutant (MT)-Trx1-overexpressing U-87MG and T98G cells. Cells were transfected with pcDNA3.1 or pcDNA3.1-TrxR1 48 h before irradiation.