Table 2. Kinetic parameters of UCH-L5_N240 derived from stopped-flow fluorescence measurements.

	k1	k2	k3	k4
(s−1)	0.14 ± 0.03	0.80 ± 0.14	10.3 ± 1.8	1120 ± 740
mf (kcal mol−1 M−1)	−1.09 ± 0.07	−0.82 ± 0.06	−0.96 ± 0.06	−1.65 ± 0.22
(s−1)	(8.1 ± 7.6)e-9	(1.9 ± 1.1)e-6	(3.1 ± 1.2)e-4	(3.1 ± 1.6)e-2
mu (kcal mol−1 M−1)	1.88 ± 0.12	1.41 ± 0.08	1.07 ± 0.05	0.81 ± 0.07
mkin (kcal mol−1 M−1)	2.97 ± 0.14	2.23 ± 0.10	2.03 ± 0.08	1.73 ± 0.14
ΔG (kcal mol−1)	9.86 ± 0.57	7.66 ± 0.36	6.16 ± 0.25	6.21 ± 0.50
[D]50% (M)	3.33 ± 0.15	3.44 ± 0.13	3.04 ± 0.10	2.52 ± 0.18