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(a) Electrophoretic pattern of pBR322 DNA (100 ng) with increasing concentration of complex 1 (5–25 μM) after 30 min incubation time in buffer (5 mMTris-HCl/50 mM NaCl, pH = 7.2 at 25 °C) (concentration dependent). (b) Cleavage pattern of pBR322 plasmid DNA (100 ng) by complex 1 (10 μM) in the presence of reactive oxygen species viz., DMSO (0.4 mM), EtOH (0.4 mM), NaN3 (0.4 mM) and SOD (10 U), after incubation for 30 min in the buffer (5 mM Tris-HCl/50 mM NaCl, pH = 7.2 at 25 °C.
