Fig. 2.

Migratory characteristics and E-cadherin expression of MCF7 cells in response to ACV treatment. a Migration of breast epithelial and MCF7 breast cancer cells through Transwell membrane. Migration percentage was counted at 3, 6 and 24 h post-seeding. b Rate of reaction of wound healing of breast epithelial cells and MCF7 breast cancer cells. c E-cadherin expression (ng/10^6 cells) in MCF7 cells treated with ACV. d Immunofluorescence staining of protein level of E-cadherin (green) in MCF7 cells after 3 and 7 days treatment. Nuclei are shown in blue. Error bars represent a 95% confidence interval based on the standard deviation. (*) indicates p <0.05 as compared with other samples and for pairwise comparison. One way ANOVA tests followed by Tukey’s test were used for statistical analysis. The data for each cell type were obtained from the same culture experiment