Table 1.

Primer sequences for RT-PCR and qRT-PCR of anthocyanin biosynthetic genes

Gene	Accession No.	Primer sequences
CHS	AF311783	F, 5′- TTGTTCGAGCTTGTCTCTGC −3′
		R, 5′- AGCCCAGGAACATCTTTGAG-3′
CHI	AB213651	F, 5′- GTCAGGCCATTGAAAAGCTC −3′
		R, 5′- CTAATCGTCAATGCCCCAAC −3′
F3H	AB289450	F, 5′- CAAGGCATGTGTGGATATGG-3′
		R, 5′- TGTGTCGTTTCAGTCCAAGG −3′
DFR	AB289448	F, 5′- AACCAACAGTCAGGGGAATG −3′
		R, 5′- TTGGACATCGACAGTTCCAG-3′
ANS	AB289447	F, 5′- TGGCGTTGAAGCTCATACTG −3′
		R, 5′- GGAATTAGGCACACACTTTGC −3′
ACTIN	AB158612	F, 5′- GGTCATTACCATTGGCTCAGA −3′
		R, 5′- CAACAAGTGATGGCTGGAATAAA −3′

CHS chalcone synthase, CHI chalcone isomerase, F3H flavanone-3-hydroxylase, DFR dihydroflavonol 4-reductase, ANS anthocyanidin synthase, ACTIN reference gene used for expression normalization

PCR conditions for RT-PCR: 95 °C (2 min) → [95 °C (20 s) → 57 °C (40 s) → 72 °C (30 s)] × 35 cycles → 72 °C (5 min) → 4 °C

PCR conditions for qRT-PCR: 95 °C (10 min) → [95 °C (30 s) → 57 °C (25 s) → 72 °C (35 s)] × 40 cycles → 95 °C (15 s) → 57 °C (30 s) → 95 °C (15 s)