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. 2017 Mar 24;4(2):ENEURO.0028-17.2017. doi: 10.1523/ENEURO.0028-17.2017

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Copyright © 2017 Buijs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — RC cuts do not affect main SCN output other than the ARC. Clear VIP innervation of the DMH (A) and PVN (B) in RC-cut animals. C, Also VIP staining to the SPZ and retrochiasmatic area dorsal to the knife cut is still intact in RC-cut animals. D, Staining of VIP afferents in the ARC, clearly visible in SHAM animals, disappear following RC cut (E). F, Likewise, NPY SCN staining, shown to be predominantly IGL derived, is not damaged by RC cuts. Pictures A–C, E, F are from RC animals, only D shows staining in an intact animal. Scale bar, 130 μm (A and F), 175 μm (B), 250 μm (C), 70 μm (D and E).