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ARC-specific AgRP staining shows intact innervation of (A) the PVN, (B) MnPO, (C) AVPV, and (D) DMH, not affected by the knife cuts. Scale bar, 175 μm (A–C) and 215 μm (D). All photomicrographs are from RC-cut animals. 3V, third ventricle; ON, optic nerve.
