Fig. 6.

Intracellular accumulation of (S)-2-aminobutyric acid and 2-ketobutyric acid in S. cerevisiae, expressing threonine deaminase SlTD, and one or two copies of mutated glutamate dehydrogenase EcGDH’. Yeasts expressing SlTD were co-transformed with either one or two copies of the mutated glutamate dehydrogenase derived from E. coli. Concentrations of (S)-2-aminobutyric acid (orange) and 2-ketobutyric acid (blue) were analyzed in the yeast pellets upon 24 h of growth in batch culture. Diamonds indicate OD₆₀₀ after 24 h of growth (all data: mean ± SD, n = 3)