Fig. 7.

Impact of l-threonine feeding on accumulation of (S)-2-aminobutyric acid and 2-ketobutyric acid. Yeasts were transformed with the gene combinations indicated on the x-axis’. Clones were grown for 24 h in selective medium fed with 1.0 g/L l-threonine. a (S)-2-Aminobutyric acid amounts accumulating in l-threonine-fed yeasts, shown as a percentage of non-fed yeasts; orange squares: intracellular (S)-2-aminobutyric acid, red circle: extracellular (S)-2-aminobutyric acid. b Absolute intracellular concentrations of (S)-2-aminobutyric acid (orange bars) and 2-ketobutyric acid (blue bars) in l-threonine fed yeasts. Diamonds in b indicate OD₆₀₀ after 24 h of growth (all data: mean ± SD, n = 3)