Fig. 8.

a Intracellular l-threonine concentrations in yeasts expressing HOM3-R2. Yeasts, either engineered for (S)-2-aminobutyric acid production (BsILVa + EcGDH’), or non-engineered yeasts, were transformed with a 2µ plasmid harboring the sequence encoding for mutated HOM3 (HOM3-R2) or they were transformed with an empty plasmid (wt). Intracellular l-threonine concentrations were analyzed as described in “Methods”. Diamonds indicate OD₆₀₀ after 24 h of growth (all data: mean ± SD, n = 3). b Intracellular (S)-2-aminobutyric acid concentrations in yeasts expressing HOM3-R2. Yeasts engineered for (S)-2-aminobutyric acid production (BsILVa + EcGDH’) were transformed with a 2µ plasmid harboring the sequence encoding for mutated HOM3 (HOM3-R2) or they were transformed with an empty plasmid (wt). Intracellular (S)-2-aminobutyric acid concentrations were analyzed as described in “Methods”. Diamonds indicate OD₆₀₀ after 24 h of growth (all data: mean ± SD, n = 3)