Table 2.
Strains
| Strains | Description | Selection marker | Replication origin/integration site |
|---|---|---|---|
| EVST20590 | Contains expression cassettes of ScCHA1 and ScGDH1′ | URA3 | ARS/CEN |
| EVST20591 | Contains expression cassettes of ScCHA1 and ScGDH3′ | URA3 | ARS/CEN |
| EVST20592 | Contains expression cassettes of ScCHA1 and EcGDH’ | URA3 | ARS/CEN |
| EVST20879 | Contains expression cassettes of ScCHA1 and BcLeuDH | URA3 | ARS/CEN |
| EVST20880 | Contains expression cassettes of ScCHA1 and BfLeuDH | URA3 | ARS/CEN |
| EVST20881 | Contains expression cassettes of ScGDH1′ and EcILVa | URA3 | ARS/CEN |
| EVST20882 | Contains expression cassettes of ScGDH3′ and EcILVa | URA3 | ARS/CEN |
| EVST20883 | Contains expression cassettes of EcGDH’ and EcILVa | URA3 | ARS/CEN |
| EVST20884 | Contains expression cassettes of EcILVa and BcLeuDH | URA3 | ARS/CEN |
| EVST20885 | Contains expression cassettes of EcILVa and BfLeuDH | URA3 | ARS/CEN |
| EVST21351 | Contains expression cassettes of ScCHA1 and SfValDH | URA3 | ARS/CEN |
| EVST21352 | Contains expression cassettes of EcILVa and SfValDH | URA3 | ARS/CEN |
| EVST21478 | Contains expression cassettes of ScCHA1, EcGDH’, NiCAR, EcALR, and BsSFP | URA3 | ARS/CEN |
| EVST21479 | Contains expression cassettes of ScCHA1, EcGDH’, NfCAR, EcALR, and BsSFP | URA3 | ARS/CEN |
| EVST21542 | Control strain with empty entry vectors | URA3 | ARS/CEN |
| EVST22605 | Contains expression cassettes of BsILVa and EcGDH’ | URA3 | ARS/CEN |
| EVST22606 | Contains expression cassettes of ScILV1 and EcGDH’ | URA3 | ARS/CEN |
| EVST22608 | Contains expression cassettes of SlTD and EcGDH’ | URA3 | ARS/CEN |
| EVST22609 | Contains expression cassette of ScHOM3-R2 | LEU2 | 2 micron |
| EVST22610 | Contains expression cassettes of BsILVa, EcGDH’, and ScHOM3-R2 | URA3 (BsILVa, EcGDH’), LEU2 (ScHOM3-R2) | ARS/CEN (BsILVa, EcGDH’) 2 micron (ScHOM3-R2) |
| EVST22615 | Contains expression cassettes of EcILVa, EcGDH’, MsCAR, MsPPTase, and EcALR | URA3 | ARS/CEN |
| EVST22837 | Contains expression cassette of SlTD and two EcGDH’ expression cassettes | URA3, HIS3 | ARS/CEN both |
| EVST22857 | Deletion of GLY1 ORF | – | – |
| EVST23406 | Deletion of GLY1 ORF Contains expression cassettes of BsILVa and EcGDH’ |
URA3 | ARS/CEN |
| EVST23407 | Deletion of GLY1 ORF Contains expression cassettes of SlTD and EcGDH’ |
URA3 | ARS/CEN |
| EVST24185 | Contains expression cassettes of BsILVa, EcGDH’, MmCAR, EcALR, and BsSFP | URA3 | ARS/CEN |
| EVST25556 | Control strain with empty plasmid | LEU2 | 2 micron |
| EVST25557 | Contains expression cassettes of BsILVa, EcGDH’, and empty plasmid | URA3 (BsILVa, EcGDH’), LEU2 | ARS/CEN (BsILVa, EcGDH’) 2 micron (empty plasmid) |
| EVST25635 | Contains expression cassettes of SlTD, EcGDH’, and empty plasmid | URA3 (BsILVa, EcGDH’), HIS3 | ARS/CEN both |
| EVST27022 | Contains expression cassettes of BsILVa, EcGDH’, MmCAR, EcALR, and BsSFP | URA3 | integrated into Chromosome XI-2 |