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. 2017 Mar 24;14:64. doi: 10.1186/s12985-017-0730-8

Table 1.

Primer sequences, annealing temperatures and amplicon lengths, obtained in nested PCR assays for SNPs in the TLR genes

Gene	GenBank Accession No.^a	SNP^b name	Primer sequences (5’-3’)		Annealing temperature [^oC]	Amplicon length (bps) ^c
TLR2	NC_000004.12	2258 G > A (rs5743708)	External	For: CGGAATGTCACAGGACAGC Rev: GGACTTTATCGCAGCTCTCAG	52	605
TLR2	NC_000004.12	2258 G > A (rs5743708)	Internal	For: GCCTACTGGGTGGAGAACCT Rev: GGCCACTCCAGGTAGGTCTT	59	340
TLR4	NG_011475	896 A > G	External	For: AAAACTTGTATTCAAGGTCTGGC	52	355
		(rs4986790)	External	Rev: TGTTGGAAGTGAAAGTAAGCCT	52	355
			Internal	For: AGCATACTTAGACTACTACCTCCATG	61	188
			Internal	Rev: AGAAGATTTGAGTTTCAATGTGGG	61	188
		1196 C > T	External	For: AGTTGATCTACCAAGCCTTGAGT	52	510
		(rs4986791)	External	Rev: GGAAACGTATCCAATGAAAAGA	52	510
			Internal	For: GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA	59	407
			Internal	Rev: ACCTGAAGACTGGAGAGTGAGTTAAATGCT	59	407
TLR9	EU170539	2848 G > A	External	For: GTCAATGGCTCCCAGTTCC	52	292
		(rs352140)	External	Rev: CATTGCCGCTGAAGTCCA	52	292
			Internal	For: AAGCTGGACCTCTACCACGA	59	177
			Internal	Rev: TTGGCTGTGGATGTTGTT	59	177

^a No., number

^b SNP, single nucleotide polymorphism

^c bps, base pairs