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. 2016 Dec 27;15(2):597–604. doi: 10.3892/mmr.2016.6078

Figure 4.

Figure 4.

Effect of TMZ on expression of miR-223 and effect of miR-223 on PAX6 3′-UTR luciferase reporter in GBM cells. (A) miR-223 levels were determined in U251 (left panel) and U118 (right panel) GBM cells treated with the indicated concentrations of TMZ for 48 h. *P<0.05 vs. untreated. (B) miR-223 binding sequence in wt-PAX6-3′UTR-luciferase reporter and mutated sequence in mut-PAX6-3′UTR-luciferase reporter. (C) Luciferase activities were measured in U251 (left panel) and U118 (right panel) GBM cells co-transfected with miR-223 mimic and the PAX6 3′-UTR luciferase reporter with wt-PAX6 or mut-PAX6 miR223-binding sequences. Cells co-transfected with SCR instead of miR-223 mimic were used as the control. The luciferase activity was expressed as fold changes to that of cells co-transfected with SCR (designated as 1). (D) Luciferase activities were measured in U251 (left panel) and U118 (right panel) GBM cells co-transfected with miR-223 antagomir and PAX6 3′-UTR luciferase reporter with wt-PAX6 or mut-PAX6 3′-UTR sequence. Cells co-transfected with SCR instead of miR-223 antagomir were used as the control. The luciferase activity was expressed as fold changes to that of cells co-transfected with SCR (designated as 1). *P<0.05 vs. SCR. TMZ, temozolomide; PAX6, paired box 6; UTR, untranslated region; GBM, glioblastoma multiforme; miR, miRNA; wt, wild type; mut, mutated; SCR, scrambled control.