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. 2016 Dec 27;15(2):597–604. doi: 10.3892/mmr.2016.6078

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of miR-223 on the mRNA level of PAX6 in GBM cells. (A) In U251 (left panel) and U118 (right panel) GBM cells, the mRNA levels of PAX6 were determined with RT-qPCR in control cells, cells transfected with scrambled control (SCR), and cells transfected with miR-223 antagomir. (B) In U251 (left panel) and U118 (right panel) GBM cells, the mRNA levels of PAX6 were determined by RT-qPCR in control cells, cells transfected with SCR and cells transfected with miR-223 mimic. The mRNA level of PAX6 was expressed as fold changes to that of control (designated as 1). *P<0.05 vs. control. PAX6, paired box 6; GBM, glioblastoma multiforme; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SCR, scrambled control; miR, miRNA.