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. 2016 Dec 27;15(2):597–604. doi: 10.3892/mmr.2016.6078

Figure 7.

Figure 7.

Effect of TMZ/miR-223/PAX6 signaling on proliferation of GBM cells. In (A) U251 and (B) U118 GBM cells with TMZ treatment (400 µM) for 48 h, cell proliferation was measured at 48 h of culture with a microplate reader-based BrdU cell proliferation ELISA kit in cells transfected with SCR, cells transfected with the empty pcDNA3.1 plasmid, cells transfected with Con-shRNA, cells transfected with miR-223 mimic, cells transfected with miR-223 antagomir, cells transfected with PAX6, cells transfected with PAX6-shRNA, cells co-transfected with miR-223 mimic and PAX6 and cells co-transfected with miR-223 antagomir and PAX-shRNA. Cell proliferation was indicated by OD values at 450 nm. aP<0.05 vs. controls (SCR, pcDNA3.1 and Con-shRNA); bP<0.05 vs. miR-223; cP<0.05 vs. miR-223 antagomir; dP<0.05 vs. PAX6; eP<0.05 vs. PAX6-shRNA; fP<0.05 vs. miR-223 + PAX6. TMZ, temozolomide; miR, miRNA; PAX6, paired box 6; GBM, glioblastoma multiforme; SCR, scrambled control; Con, control; shRNA, short hairpin RNA; OD, optical density.