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. 2016 Dec 22;15(2):689–695. doi: 10.3892/mmr.2016.6065

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Tim-3 is overexpressed in CRC tissues and in cultured CRC cell lines. Tissues from 30 patients with CRC were dissected and used to extract total RNA and proteins. (A) Western blot analysis revealed that the protein levels of Tim-3 were significantly higher in the clinical CRC samples, compared with the paired non-cancerous samples. GAPDH was included as an inner control. *P<0.05. (B) Total RNAs from the 30 clinical CRC samples and adjacent tissues were subjected to reverse transcription-quantitative polymerase chain reaction analysis. It was shown that the mRNA levels of Tim-3 were higher in the CRC tissues, compared with the adjacent non-cancerous tissues. (C) Immunohistochemical analysis was performed in slides from 112 clinical cases of CRC. For each case, the staining intensity of Tim-3 was classified as low (upper panel) or high (lower panel) expression (magnificantion, ×400). (D) Differential expression of Tim-3 was shown in CRC cell lines. The 293T cell line was included as a control. Tim-3, T cell immunoglobulin mucin-3; CRC, colorectal carcinoma; Adj, adjacent non-cancerous tissue.