Table I.
Primers used for Sanger sequencing of the SLC12A1 gene.
| Exon | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
| 2 | 5′-AGCTCCCTAATGGAAGCACA-3′ | 5′-GTAAGTAAGATGGATAGTGTTT-3′ | 531 |
| 3 and 4 | 5′-TCAATTGTTTTGATTTGCTTTGA-3′ | 5′-TGCTTATTGAATTTATGATTTACATGC-3′ | 507 |
| 5 | 5′-GAGGCATGGACCTGAAAACT-3′ | 5′-GCATCCAGCTCCTCCAAATA-3′ | 337 |
| 6 | 5′-TCAGATAGTCACAATCGTTTGGTT-3′ | 5′-TCCCTTAGTGCCCTGAGAAG-3′ | 289 |
| 7 | 5′-CCTATATGGCCCCAGGTGTA-3′ | 5′-TGCCTGCTCATTTCACCATA-3′ | 351 |
| 8 | 5′-TCTGGGTAGCAGAGACTTAACTGA-3′ | 5′-TGATGGGGATGGTGATGAG-3′ | 311 |
| 9 | 5′-GGACTAGGGAAGCCAATGGT-3′ | 5′-TTTGAATCTGTAGGGTAATATGGTCA-3′ | 318 |
| 10 | 5′-CATCAACTTGCTGTTTGCTTG-3′ | 5′-TGCTGCATTGAAAGCTCACT-3′ | 313 |
| 11 | 5′-CAGAGGCTAAGAAATGGACCTT-3′ | 5′-GCCAATAATCAATCAGTTGCAG-3′ | 363 |
| 12 | 5′-CTGGGCGATAGAGCGAGACT-3′ | 5′-ACTTGAGCCAGATGCAAACA-3′ | 401 |
| 13 | 5′-TCCCCAAATCTTCTTGTTTGA-3′ | 5′-CAAACTAAAAGGAAAGCCCTATGA-3′ | 286 |
| 14 | 5′-TGACAGATGCTCGCTATGTTTT-3′ | 5′-GGATTACGCTTCCCACTAGG-3′ | 352 |
| 15 | 5′-AGATTCTGGAACTTGGCCTAAA-3′ | 5′-ATCCACCTTACATATGCCTCA-3′ | 401 |
| 16 | 5′-ATTTGGGCACTCATCTTTGC-3′ | 5′-TTTGTATGACTGCTTATTTTTAGAATG-3′ | 354 |
| 17 | 5′-GAGAGGTTGCCCCATTTTTC-3′ | 5′-GCATGTACCTTTTCTCCTCACC-3′ | 306 |
| 18 | 5′-GGTCATCTCCAAAAGGCTGA-3′ | 5′-TGCTTGGCTGCCTAACTACA-3′ | 413 |
| 19 | 5′-TCAGGATCTTCAGAACATTACTTCA-3′ | 5′-AAGTCAGAGTACCAGGGGAATG-3′ | 353 |
| 20 | 5′-AAATGCATCAGCTCTTGGCTA-3′ | 5′-GCCCTGCTGTATCCCATAAC-3′ | 310 |
| 21 | 5′-GGTGATTTTGTCTTCTTTCATCA-3′ | 5′-TGGAGAGAAACCTTTCAGTTCCT-3′ | 301 |
| 22 | 5′-TTGTTTCTGCCCTCAAAAGC-3′ | 5′-TCCCATATACCTTCTCATGCAGT-3′ | 240 |
| 23 | 5′-ACTTAATTAAGAGCTATCAA-3′ | 5′-ATTAAAGCAACAAACCTCTGAAATG-3′ | 270 |
| 24 | 5′-CCAACCAAAAAGCCTCTGTC-3′ | 5′-TCAAGAAGTCGCTGCAGTAAA-3′ | 336 |
| 25 | 5′-GCATGATATTCAGCTCTGATTCC-3′ | 5′-TGAAAAATGCAGAAAGCTTGG-3′ | 385 |
| 26 | 5′-AAACACCATAAGTTTCTAAGCCTGA-3′ | 5′-CCTGAAGAGTCCCAAGCTTTT-3′ | 308 |
| 27 | 5′-GCTCAGAAATACTAGTGCCGTTA-3′ | 5′-TCCTCTCCAGAGGTTTGCAT-3′ | 324 |
Primers highlighted in bold were used for the long-range PCR assay. All the primers were designed in the introns and covered intron/exon junctions. The primers were designed to have polymerase chain reaction products covering each exon and a minimum 20 bp flanking region in the introns. SLC12A1, solute carrier family 12 member 1.