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. 2016 Dec 29;15(2):613–626. doi: 10.3892/mmr.2016.6083

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Copyright: © Xiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — DMN and YGD regulate the differentiation of BMSCs into hepatocytes and biliary epithelial cells. Mice were injected with DMN or normal saline for 4 weeks. Following 4 weeks of DMN treatment, mice were treated with YGD or HGF for a further 4 weeks. (A) The expression of CD90 (green, top row) and Alb (red, middle row) was detected by immunofluorescence, with the co-localization analyzed by merging these images (bottom row). (B) The expression of CD90 (green, top row) and CK18 (red, middle row) was detected by immunofluorescence, with the co-localization analyzed by merging these images (bottom row). Expression and co-localization was increased by DMN injection and further increased by YGD treatment. Magnification, ×60. YGD, Yi Guan Jian decoction; HGF, hepatocyte growth factor; DMN, dimethylnitrosamine; CD, cluster of differentiation; Alb, Albumin; CK18, cytokeratin 18; C, control; M, model; W, week.