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. 2016 Dec 30;15(2):567–572. doi: 10.3892/mmr.2016.6098

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Copyright: © Ling et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Resveratrol induces adventitial fibroblast apoptosis. (A) Flow cytometric analysis of cell apoptosis following resveratrol treatment. Q4 represents the ratio of apoptotic cells. (B) Statistical analysis of the results. High-dose resveratrol induced cell apoptosis of adventitial fibroblasts (control, vs. 80 µmol/l group; *P<0.05). This pro-apoptotic effect was partially reversed by siRNA transfection. The apoptotic ratio was significantly decreased in the 80 µmol/l+siRNA group, compared with the 80 µmol/l resveratrol group (*P<0.05). There was a significant difference between the 80 µmol/l+siRNA group, compared with the control group (*P<0.05). Res, resveratrol; siRNA, small interfering RNA; SIRT1, sirtuin 1; PE, phycoerythrin; FITC, fluorescein isothiocyanate.