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. 2016 Dec 14;15(2):703–711. doi: 10.3892/mmr.2016.6038

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — The purity, titer and transfection efficiency of rAAV9-shRNA-Adrb1-ZsGreen vectors in HEK293 cells. (A) The purity of rAAV9-shRNA-Adrb1-ZsGreen vectors as determined by SDS-PAGE followed by Coomassie Brilliant Blue staining. The left lane indicates the protein maker (1), and the right lane indicates the purified vector sample (2). (B) The titer of the purified vectors. Spots 1–8 indicate 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78, and 0.39×10⁹ v.g./ml purified vector, respectively (a, 2-fold dilution of purified vectors; b, purified vectors). (C) Fluorescence (left) and light (right) microscope images of the transfection efficiency of rAAV9-shRNA-Adrb1-ZsGreen vectors in HEK293 cells (magnification, ×200). rAAV9, recombinant adeno-associated virus type 9 vector; shRNA, short-hairpin RNA; Adrb1, adrenergic receptor β1; v.g., viral genomes.