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. 2016 Dec 22;15(2):759–767. doi: 10.3892/mmr.2016.6066

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Changes in the number of leukocytes and in the hematopoietic function of mice irradiated with γ-radiation. The function of the hematopoietic system was examined to test the radiosensitivity of IRM-2 mice. IRM-2, ICR/JCL and 615 mice were irradiated with 4 Gy γ-radiation (n=8). (A) Whole blood was extracted from the orbital sinuses on 2, 5, 9, 15 and 21 days after irradiation and the leukocytes were counted. *P<0.05 vs. IRM-2 mice 12 to 21 days after radiation. (B) Whole blood was extracted from the orbital sinuses on 2, 5, 9, 15 and 21 days after irradiation and the BMNCs were counted. (C) BMCs were flushed from mouse femurs on 2, 5, 9, 15 and 21 days after irradiation and treated with a 0.2 mol/l HClO₄ solution. The DNA content of the BMCs was measured at 286 nm. (D) Spleens were harvested from the abdominal cavity of mice on 2, 5, 9, 15 and 21 days after irradiation and CFU-S was counted under a microscope. *P<0.05, **P<0.01 vs. IRM-2 mice 9 to 21 days after radiation. BMNCs, bone marrow nucleated cells; BMCs, bone marrow cells; CFU-S, colony-forming unit-spleen.