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Nampt does not affect the bone-resorbing activity of mature osteoclasts. Mature osteoclasts were seeded on hydroxyapatite-coated plates and treated for 24 h with Nampt (500 ng/ml). Attached cells were removed and images were captured under a light microscope (magnification, ×5). Pit areas on the hydroxyapatite plate were quantified using the Image Pro-Plus (version 4.5) software. Nampt, nicotinamide phosphoribosyltransferase.
