Figure 4.

Slit2 decreased TNF-α-induced cell proliferation and migration in RAECs. (A and B) RAEC migration was detected by Transwell analysis (magnification, ×100). RAEC migratory ability was improved by 10 ng/ml TNF-α; however, Slit2 attenuated the increase in migratory ability. As the added concentration of Slit2 increased, the TNF-α-induced migratory ability of RAECs was markedly inhibited. (C) RAEC proliferation was increased by 10 ng/ml TNF-α; however, Slit2 ameliorated proliferation in a dose-dependent manner. *P<0.05 vs. control group; ▲P<0.05 vs. TNF-α group; ^◊P<0.05 vs. 25 ng/ml Slit2 group; ▼P<0.05 vs. 50 ng/ml Slit2 group. RAECs, rat aortic endothelial cells; TNF-α, tumor necrosis factor-α; Slit2, Slit homolog 2.