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. 2017 Feb 22;15(4):1981–1988. doi: 10.3892/mmr.2017.6240

Figure 6.

Figure 6.

Inhibitory effects of Slit2 on TNF-α-induced cell proliferation and migration were mediated via the VEGF-Notch pathway. (A) VEGF levels. RAECs were treated with 10 ng/ml TNF-α or 100 ng/ml Slit2, in the presence or absence of VEGF siRNA (200 nM). TNF-α induced an increase in VEGF levels; however, Slit2 and VEGF siRNA reduced the expression levels of VEGF [*P<0.05 vs. TNF-α(+)/Slit2(−)/VEGF(−)/NS siRNA (−)-transfected cells]. (B) Notch1 and Notch2 protein expression levels were markedly decreased following VEGF siRNA transfection. (C) RAEC proliferation. Slit2 and VEGF siRNA transfection inhibited TNF-α-induced cell proliferation [♦P<0.05 vs. TNF-α(+)/Slit2(−)/VEGF(−)/NS siRNA(−)-transfected cells]. (D) RAEC migration. Slit2 and VEGF siRNA transfection inhibited TNF-α-induced cell migration [♦P<0.05 vs. TNF-α(+)/Slit2(−)/VEGF(−)/NS siRNA(−)-transfected cells]. RAECs, rat aortic endothelial cells; TNF-α, tumor necrosis factor-α; Slit2, Slit homolog 2; VEGF, vascular endothelial growth factor; NS, non-specific.