Table VI.
Selected gene validation results by RT-qPCR with corresponding microarray results for comparison.
| Hair follicle vs. skin | Basal cell carcinomas vs. skin | |||||||
|---|---|---|---|---|---|---|---|---|
| Microarray | RT-qPCR | Microarray | RT-qPCR | |||||
| Gene name | Fold change | q-value | Fold change | P-value | Fold change | q-value | Fold change | P-value |
| NOTCH1 | 4.07350142 | 8.64548474 | 89.7796173 | 3.0878E-08 | 2.25963492 | 55.1681869 | 3.2667374 | 0.01731453 |
| NOTCH2 | 0.49022594 | 0.39254973 | 94.051046 | 2.4841E-06 | 0.46324991 | 2.15276566 | 0.87297527 | 0.84563107 |
| NOTCH4 | 2.69005573 | 0.24322855 | 19.2601231 | 0.00128485 | 1.71844966 | 55.1681869 | 2.67459359 | 0.14313619 |
| DTX1 | 1.17722862 | 48.8237978 | 16.3788042 | 0.03308844 | 2.4295169 | 55.1681869 | 0.28089744 | 0.22707567 |
| DTX2 | 0.99256711 | 40.6530129 | 18.9813992 | 0.00157923 | 0.47607531 | 4.07129257 | 0.43629239 | 0.08540183 |
| LNX1 | – | – | 198.293295 | 3.6156E-08 | 1.72295346 | 55.1681869 | 2.072439 | 0.28247621 |
| DVL2 | 13.8816522 | 3.95106797 | 16.104213 | 0.00293444 | 1.55096741 | 55.1681869 | 2.94822536 | 0.03581873 |
| NUMB | 3.28845002 | 0.48722077 | 182.325561 | 4.9233E-06 | 1.30366339 | 55.1681869 | 1.33412783 | 0.63444821 |
| JAG1 | 0.34149885 | 0.18592759 | 365.28094 | 2.3222E-08 | 1.63466178 | 55.1681869 | 1.83632659 | 0.35967923 |
| JAG2 | 1.5839265 | 41.2725397 | 179.447584 | 8.3358E-05 | 1.33391274 | 55.1681869 | 0.31170539 | 0.23588105 |
| LFNG | 2.07190237 | 6.92879545 | 1889.72022 | 4.5482E-05 | 2.33909215 | 55.1681869 | 1.52408184 | 0.61264104 |
| KRT17 | 3.021 | 0.00846 | 27272.867 | 2.2916E-09 | 7.21676553 | 55.1681869 | 51.4001016 | 0.00100685 |
| HES1 | – | – | 1067.11032 | 1.053E-15 | 0.76047244 | 67.4104045 | 2.91116399 | 0.0400455 |
| HES7 | 1.19000588 | 48.8237978 | 3262.24091 | 1.5035E-08 | 4.16958086 | 29.7263863 | 0.29241452 | 0.13032618 |
| HR | 2.16394516 | 2.23319493 | 226.55094 | 1.0638E-09 | 1.09510072 | 58.338051 | 1.19521471 | 0.76942586 |
| RBPSUHL | 3.11300222 | 41.2725397 | 255.196596 | 7.7485E-10 | 1.22074356 | 55.1681869 | 0.16526889 | 0.06136225 |
| DAAM1 | 0.20611879 | 0.21537795 | 574.06641 | 1.5066E-07 | 0.54865054 | 18.0863137 | 1.63754261 | 0.55059198 |
| MAPK8 | 1.5501382 | 42.0929177 | 199.824645 | 1.1302E-06 | 0.85527761 | 67.7038487 | 0.96727335 | 0.97161104 |
| GLI1 | – | – | 52.4313289 | 0.00072822 | – | – | 1.87408166 | 0.51779197 |
| GLI2 | 2.52604018 | 22.3203103 | 319.860846 | 8.9511E-07 | 2.00283366 | 55.1681869 | 12.2544249 | 0.00422916 |
| RAC1 | 0.26859543 | 1.21189849 | 535.384925 | 2.0661E-11 | 0.65505096 | 61.6040693 | 1.31400567 | 0.66627414 |
| ROCK2 | 0.18494564 | 2.62913482 | 575.373988 | 8.4351E-07 | 0.82910219 | 67.7038487 | 1.38128144 | 0.6297566 |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction.