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. 2017 Feb 3;15(4):1673–1681. doi: 10.3892/mmr.2017.6165

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Osteogenic differentiation markers analysis in DKK3-shRNA DFCs in vitro. (A) ALP activity of DKK3-shRNA DFCs was enhanced compared with in vector-infected DFCs between days 7 and 21. (B) General view of mineralized nodules of DKK3-shRNADFCs following growth in mineral-induction medium for 3 weeks. (C) General view of mineralized nodules of vector-infected DFCs following mineral induction for 3 weeks. (D) Calcified nodules of DKK3-shRNADFCs under microscopy. Scale bar, 200 µm. (E) Calcified nodules of vector-infected DFCs under microscopy. Scale bar, 200 µm. (F) mRNA expression of Col-I was upregulated in DKK3-shRNA DFCs grown in mineral-induction medium. (G) mRNA expression of ALP was upregulated in DKK3-shRNA DFCs grown in mineral-induction medium. (H) WB analysis demonstrating nuclear β-catenin expression inDKK3-shRNADFCs grown in mineral-induction medium. (I) Relative gray scale of WB analysis results in (H). (J) WB analysis of DKK3, RUNX2, OCN and β-catenin expression in DKK3-shRNADFCs grown in mineral-induction medium. (K-N) Relative gray scale of WB analysis results in (J). *P<0.05, **P<0.01 vs. vector-infected DFCs. ALP, alkaline phosphatase; DKK3, Dickkopf-related protein 3; ShRNA/sh, short hairpin RNA; DFCs, dental follicle cells; Col-I, collagen-type-I; WB, western blot; RUNX2, runt-related transcription factor 2; OCN, osteocalcin.