Table I.
Wnt polymerase chain reaction array results of dental follicle cells cultured in mineral induction medium or 10% Dulbecco's modified Eagle's medium for 4 weeks.
| Average ∆∆Cq | 2−∆∆Cq | t-test | Fold up or downregulation | |||
|---|---|---|---|---|---|---|
| Gene | Mineral 4 weeks | 10% DMEM 4 weeks | Mineral 4 weeks | 10% DMEM 4 weeks | P-value | Mineral 4 weeks/10% DMEM 4 weeks |
| Apc | 5.88 | 5.68 | 1.7E-02 | 2.0E-02 | 0.716282 | −1.15 |
| Apc2 | 11.35 | 10.39 | 3.8E-04 | 7.5E-04 | 0.715525 | −1.95 |
| Axin1 | 6.93 | 6.93 | 8.2E-03 | 8.2E-03 | 0.854303 | 1.00 |
| Axin2 | 8.82 | 9.12 | 2.2E-03 | 1.8E-03 | 0.570925 | 1.23 |
| Bcl9 | 6.56 | 5.89 | 1.1E-02 | 1.7E-02 | 0.273815 | −1.59 |
| Btrc | 6.53 | 6.17 | 1.1E-02 | 1.4E-02 | 0.198065 | −1.28 |
| Ctnnb1 | 2.80 | 2.48 | 1.4E-01 | 1.8E-01 | 0.794771 | −1.25 |
| Ccnd1 | 2.20 | 2.38 | 2.2E-01 | 1.9E-01 | 0.520418 | 1.13 |
| Ccnd2 | 4.18a | 3.07a | 5.5E-02a | 1.2E-01a | 0.031100 | −2.15a |
| Ccnd3 | 4.22 | 3.92 | 5.3E-02 | 6.6E-02 | 0.719887 | −1.24 |
| Csnk1a1 | 3.29 | 2.99 | 1.0E-01 | 1.3E-01 | 0.476922 | −1.23 |
| Csnk1d | 4.32 | 4.50 | 5.0E-02 | 4.4E-02 | 0.475343 | 1.13 |
| Csnk2a1 | 4.52 | 4.48 | 4.3E-02 | 4.5E-02 | 0.940791 | −1.03 |
| Csnk2b | 3.71 | 3.69 | 7.6E-02 | 7.7E-02 | 0.940995 | −1.02 |
| Daam1 | 6.20 | 6.04 | 1.4E-02 | 1.5E-02 | 0.632151 | −1.12 |
| Dixdc1 | 8.45 | 7.99 | 2.9E-03 | 3.9E-03 | 0.971372 | −1.38 |
| Dkk1 | 11.79 | 12.24 | 2.8E-04 | 2.1E-04 | 0.414675 | 1.37 |
| Dkk3 | 3.15a | 2.21a | 1.1E-01a | 2.2E-01a | 0.049257a | −1.91a |
| Dkk4 | 7.09 | 6.59 | 7.3E-03 | 1.0E-02 | 0.299526 | −1.42 |
| Dvl1 | 6.15 | 6.02 | 1.4E-02 | 1.5E-02 | 0.955741 | −1.10 |
| Dvl2 | 4.48 | 4.26 | 4.5E-02 | 5.2E-02 | 0.426044 | −1.17 |
| Dvl3 | 7.08a | 6.09a | 7.4E-03a | 1.5E-02a | 0.011969a | −1.99a |
| Ep300 | 6.05 | 5.82 | 1.5E-02 | 1.8E-02 | 0.976422 | −1.17 |
| Fbxw11 | 4.56 | 4.29 | 4.2E-02 | 5.1E-02 | 0.585973 | −1.21 |
| Fbxw2 | 3.24 | 3.16 | 1.1E-01 | 1.1E-01 | 0.902830 | −1.06 |
| Fgf4 | 13.88 | 13.19 | 6.6E-05 | 1.1E-04 | 0.154326 | −1.61 |
| Frzb | 13.59 | 13.02 | 8.1E-05 | 1.2E-04 | 0.541255 | −1.48 |
| Fzd1 | 2.62 | 1.65 | 1.6E-01 | 3.2E-01 | 0.537557 | −1.96 |
| Fzd2 | 3.83 | 2.76 | 7.0E-02 | 1.5E-01 | 0.200846 | −2.09 |
| Fzd3 | 6.90 | 6.93 | 8.4E-03 | 8.2E-03 | 0.843733 | 1.02 |
| Fzd4 | 6.28 | 6.94 | 1.3E-02 | 8.2E-03 | 0.405254 | 1.58 |
| Fzd5 | 8.85 | 8.44 | 2.2E-03 | 2.9E-03 | 0.233498 | −1.32 |
| Fzd6 | 6.30 | 6.26 | 1.3E-02 | 1.3E-02 | 0.744854 | −1.03 |
| Fzd7 | 6.83 | 5.46 | 8.8E-03 | 2.3E-02 | 0.155697 | −2.59 |
| Fzd9 | 14.13 | 13.93 | 5.6E-05 | 6.4E-05 | 0.941213 | −1.15 |
| Gsk3a | 3.11 | 3.08 | 1.2E-01 | 1.2E-01 | 0.947278 | −1.02 |
| Gsk3b | 3.89 | 3.59 | 6.8E-02 | 8.3E-02 | 0.388341 | −1.23 |
| Jun | 5.31 | 4.74 | 2.5E-02 | 3.7E-02 | 0.579975 | −1.48 |
| Kremen1 | 5.85 | 5.63 | 1.7E-02 | 2.0E-02 | 0.540391 | −1.17 |
| Lef1 | 10.74 | 10.72 | 5.8E-04 | 5.9E-04 | 0.832187 | −1.02 |
| Lrp5 | 6.78 | 7.04 | 9.1E-03 | 7.6E-03 | 0.489102 | 1.19 |
| Lrp6 | 3.81 | 3.37 | 7.1E-02 | 9.7E-02 | 0.734114 | −1.36 |
| Mitf | 6.57 | 7.00 | 1.1E-02 | 7.8E-03 | 0.318318 | 1.35 |
| Myc | 4.65 | 5.27 | 4.0E-02 | 2.6E-02 | 0.311155 | 1.53 |
| Nkd1 | 7.24 | 6.07 | 6.6E-03 | 1.5E-02 | 0.145820 | −2.25 |
| Nkd2 | 4.78 | 4.29 | 3.6E-02 | 5.1E-02 | 0.451828 | −1.40 |
| RGD1561440 | 6.47 | 6.24 | 1.1E-02 | 1.3E-02 | 0.437063 | −1.17 |
| Pitx2 | 10.00 | 10.03 | 9.8E-04 | 9.6E-04 | 0.857792 | 1.02 |
| Porcn | 4.75 | 5.34 | 3.7E-02 | 2.5E-02 | 0.415860 | 1.50 |
| Ppp2ca | 3.52 | 3.35 | 8.7E-02 | 9.8E-02 | 0.652950 | −1.12 |
| Ppp2r1a | 2.99 | 3.10 | 1.3E-01 | 1.2E-01 | 0.611666 | 1.08 |
| Pygo2 | 6.82 | 6.55 | 8.9E-03 | 1.1E-02 | 0.917801 | −1.20 |
| Rhoa | 0.82 | 0.98 | 5.7E-01 | 5.1E-01 | 0.451892 | 1.12 |
| Senp2 | 5.13 | 4.95 | 2.9E-02 | 3.2E-02 | 0.606892 | −1.13 |
| Sfrp1 | 4.83 | 3.51 | 3.5E-02 | 8.8E-02 | 0.434107 | −2.49 |
| Sfrp2 | 6.31 | 6.01 | 1.3E-02 | 1.6E-02 | 0.470746 | −1.23 |
| Sfrp4 | 7.43 | 7.00 | 5.8E-03 | 7.8E-03 | 0.885189 | −1.34 |
| Sfrp5 | 11.47 | 11.58 | 3.5E-04 | 3.3E-04 | 0.786232 | 1.08 |
| Tcf3 | 6.38 | 6.24 | 1.2E-02 | 1.3E-02 | 0.618723 | −1.10 |
| Tcf4 | 4.21 | 4.13 | 5.4E-02 | 5.7E-02 | 0.699509 | −1.06 |
| Tcf7 | 11.27 | 10.70 | 4.1E-04 | 6.0E-04 | 0.216594 | −1.48 |
| Tcfe2a | 6.00 | 5.55 | 1.6E-02 | 2.1E-02 | 0.505454 | −1.36 |
| Tle1 | 7.21 | 6.69 | 6.8E-03 | 9.7E-03 | 0.770935 | −1.43 |
| Tle2 | 7.22 | 6.54 | 6.7E-03 | 1.1E-02 | 0.529451 | −1.60 |
| Wif1 | 7.16 | 7.56 | 7.0E-03 | 5.3E-03 | 0.328635 | 1.32 |
| Wisp1 | 2.52 | 1.95 | 1.7E-01 | 2.6E-01 | 0.586035 | −1.49 |
| Wnt1 | 13.16 | 12.49 | 1.1E-04 | 1.7E-04 | 0.374245 | −1.59 |
| Wnt10a | 13.75 | 13.27 | 7.2E-05 | 1.0E-04 | 0.948650 | −1.40 |
| Wnt10b | 13.09 | 12.90 | 1.1E-04 | 1.3E-04 | 0.748260 | −1.14 |
| Wnt11 | 8.51 | 7.61 | 2.8E-03 | 5.1E-03 | 0.287261 | −1.86 |
| Wnt2 | 11.62 | 10.83 | 3.2E-04 | 5.5E-04 | 0.373755 | −1.73 |
| Wnt2b | 10.97 | 10.77 | 5.0E-04 | 5.7E-04 | 0.634100 | −1.15 |
| Wnt3 | 10.48 | 10.23 | 7.0E-04 | 8.3E-04 | 0.564868 | −1.19 |
| Wnt3a | 6.61 | 6.06 | 1.0E-02 | 1.5E-02 | 0.540905 | −1.46 |
| Wnt4 | 8.59 | 8.88 | 2.6E-03 | 2.1E-03 | 0.869003 | 1.23 |
| Wnt5a | 3.42 | 3.69 | 9.4E-02 | 7.8E-02 | 0.588542 | 1.21 |
| Wnt5b | 5.54 | 5.31 | 2.1E-02 | 2.5E-02 | 0.402236 | −1.17 |
| Wnt6 | 15.60 | 16.84 | 2.0E-05 | 8.5E-06 | 0.332610 | 2.35 |
| Wnt7a | 17.12 | 17.92 | 7.0E-06 | 4.0E-06 | 0.415976 | 1.74 |
| Wnt7b | 7.46 | 6.70 | 5.7E-03 | 9.6E-03 | 0.243376 | −1.70 |
| Wnt8a | 14.74 | 13.88 | 3.6E-05 | 6.6E-05 | 0.276427 | −1.82 |
| Wnt8b | 16.05 | 16.15 | 1.5E-05 | 1.4E-05 | 0.502530 | 1.07 |
| Wnt9a | 8.94 | 8.93 | 2.0E-03 | 2.1E-03 | 0.497268 | −1.01 |
| Wnt9b | 9.38 | 9.38 | 1.5E-03 | 1.5E-03 | 0.642663 | 1.00 |
| Rplp1 | −2.04 | −2.43 | 4.1E+00 | 5.4E+00 | 0.058664 | −1.31 |
| Hprt1 | 4.43 | 4.61 | 4.6E-02 | 4.1E-02 | 0.526594 | 1.13 |
| Rpl13a | −2.39 | −2.18 | 5.2E+00 | 4.5E+00 | 0.372639 | 1.16 |
| Ldha | 2.88 | 2.93 | 1.4E-01 | 1.3E-01 | 0.431255 | 1.03 |
| Actb | −3.08 | −2.86 | 8.5E+00 | 7.3E+00 | 0.536666 | 1.16 |
a
P<0.05 vs. control.