Table I.
Gene | PCR amplification fragment length (bp) | Primer sequence (5′→3′) |
---|---|---|
β-actin | 231 | F: 5′-ACTGCCCTGGCTCCTAGCA-3′ |
R: 5′-GCCAGGATAGAGCCACCAATC-3′ | ||
TLR4 | 100 | F: 5′-GCATCATCTTCATTGTCCTTGAGA-3′ |
R: 5′-CTCCCACTCGAGGTAGGTGTTT-3′ | ||
TLR2 | 285 | F: 5′-CGCTTCCTGAACTTGTCC-3′ |
R: 5′-GGTTGTCACCTGCTTCCA-3′ |
PCR, polymerase chain reaction; TLR 4, Toll-like receptor 4; TLR 2, Toll-like receptor 2; F, forward; R, reverse.