Table II.

PCR amplification system.

Agent	Volume (µl)
2X Taq PCR Master Mix	10
P-f (10 pmol/µl)	0.3
P-r (10 pmol/µl)	0.3
DNA template	2.0
ddH2O	7.4

PCR, polymerase chain reaction; ddH₂O, double distilled water; P-f, forward primer; P-r, reverse primer.