Table IV.
Agent | Volume (µl) |
---|---|
2X SYBR® Premix Ex Taq™ | 10 |
P-f (10 pmol/µl) | 0.3 |
P-r (10 pmol/µl) | 0.3 |
DNA template | 2.0 |
ddH2O | 7.4 |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ddH2O, double distilled water; P-f, forward primer; P-r, reverse primer.