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RT-qPCR reaction system.
| Agent | Volume (µl) |
|---|---|
| 2X SYBR® Premix Ex Taq™ | 10 |
| P-f (10 pmol/µl) | 0.3 |
| P-r (10 pmol/µl) | 0.3 |
| DNA template | 2.0 |
| ddH2O | 7.4 |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ddH2O, double distilled water; P-f, forward primer; P-r, reverse primer.
